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Journal: bioRxiv
Article Title: Universal nucleic acid preservation in biological fluids with boron clusters
doi: 10.64898/2026.01.13.697153
Figure Lengend Snippet: a Human plasma experiment schematic. b Plasma λ DNA levels were measured at various time-points (0, 1, 7 and 14 days) by real-time PCR (n=3). Final plasma concentration of [B 12 H 12 ] 2- is 0.25 M, and the initial λ DNA spike-in concentration in plasma is as indicated. Bars represent group mean ± SD. c Plasma MS2 ssRNA levels were measured at various time-points (0, 1, 3, 7 and 14 days) by real-time PCR (n=3). Final plasma concentrations of [B 12 H 12 ] 2- , SUPERase.In and RNase inhibitor are 0.25 M, 2U/μL and 4U/μL, respectively. The initial MS2 ssRNA spike-in concentration in plasma is as indicated. Bars represent group mean ± SD. d Human urine experiment schematic. e Urine λ DNA levels were measured at various time-points (0, 1, 3, 7 and 14 days) by real-time PCR (n=3). Final urine concentration of [B 12 H 12 ] 2- is 0.25 M, and the initial λ DNA spike-in concentration in urine is as indicated. Bars represent group mean ± SD. f Urine MS2 ssRNA levels were measured at various time-points (0, 1, 3, 7 and 14 days) by real-time PCR (n=3). Final urine concentrations of [B 12 H 12 ] 2- , SUPERase.In and RNase inhibitor are 0.25 M, 2U/μL and 4U/μL, respectively. The initial MS2 ssRNA spike-in concentration in urine is as indicated. Bars represent group mean ± SD.
Article Snippet: RNase inhibitor was purchased from Molecular Cloning Laboratories (cat no. RNIN200). λ DNA-HindIII Digest (cat no. N3012) and dsRNA Ladder (cat no. N0363S) were purchased from New England BioLabs.
Techniques: Clinical Proteomics, Real-time Polymerase Chain Reaction, Concentration Assay